FYN antibody (N-Term)

Catalog No. ABIN184620

The Goat Polyclonal anti-FYN antibody (ABIN184620) specifically detects FYN in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for FYN antibody (N-Term) (ABIN184620)

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FYN antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-FYN Antibody

Binding Specificity: N-Term

Purpose: FYN

Sequence: VQCKDKEATK LTE

Specificity: This antibody is expected to recognise all three reported isoforms (as represented by NP_002028.1, NP_694592.1, NP_694593.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence VQCKDKEATKLTE, from the N Terminus of the protein sequence according to NP_002028.1, NP_694592.1, NP_694593.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in lysates of cell lines A431 and Jurkat (calculated MW of 54.5 kDa according to NP_694593.1). Recommended for use at 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FYN

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Alternative Name: FYN

Background: FYN, SLK, SYN, MGC45350, FYN oncogene related to SRC, FGR, YES, src-like kinase, c-syn protooncogene, tyrosine kinase p59fyn(T), src/yes-related novel gene, OKT3-induced calcium influx regulator, proto-oncogene tyrosine-protein kinase fyn, RP1-66H14.1, OT

Gene ID: 2534, 14360, 25150

NCBI Accession: NP_002028, NP_694592, NP_694593

Pathways: JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling