PYCARD antibody (C-Term)

Catalog No. ABIN184674

This anti-PYCARD antibody is a Goat Polyclonal antibody detecting PYCARD in WB, ELISA and IHC. Suitable for Human.

Quick Overview for PYCARD antibody (C-Term) (ABIN184674)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PYCARD Antibody

Binding Specificity: C-Term

Purpose: ASC / TMS1

Sequence: RESQSYLVED LERS

Specificity: This antibody is expected to recognise both reported isoforms (NP_037390.2 and NP_660183.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RESQSYLVEDLERS, from the C Terminus of the protein sequence according to NP_037390.2, NP_660183.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows staining of sinusoidal lining cells. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 19 kDa band observed in lysates of cell line HeLa (calculated MW of 20.0 kDa according to NP_660183.1). Recommended concentration: 0.2-0.6 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: PYCARD

Background: PYCARD, ASC, TMS1, PYD and CARD domain containing, CARD5, MGC10332, TMS, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1, TMS-1

Gene ID: 29108

NCBI Accession: NP_037390, NP_660183

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome