NUMB antibody (C-Term)

Catalog No. ABIN184679

The Goat Polyclonal anti-NUMB antibody has been validated for IHC, ELISA, IF and FACS. It is suitable to detect NUMB in samples from Human and Mouse.

Quick Overview for NUMB antibody (C-Term) (ABIN184679)

Target: NUMB (Numb Homolog (NUMB))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This NUMB antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-NUMB Antibody

Binding Specificity: C-Term

Purpose: NUMB

Sequence: PFSSDLQKTF EIEL

Specificity: This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet b

Cross-Reactivity: Cow, Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PFSSDLQKTFEIEL, from the C Terminus of the protein sequence according to NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 6-8 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cell junctions/plasma membrane and cytoplasm of A431 and NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NUMB

Target: NUMB (Numb Homolog (NUMB))

Alternative Name: NUMB

Background: NUMB, numb homolog (Drosophila), S171, numb homolog

Gene ID: 8650, 18222

NCBI Accession: NP_001005743, NP_003735, NP_001005745

Pathways: Cell-Cell Junction Organization