RAC2 antibody (C-Term)

Catalog No. ABIN184689

The Goat Polyclonal anti-RAC2 antibody (ABIN184689) specifically detects RAC2 in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for RAC2 antibody (C-Term) (ABIN184689)

Target: RAC2 (Ras-Related C3 Botulinum Toxin Substrate 2 (Rho Family, Small GTP Binding Protein Rac2) (RAC2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RAC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-RAC2 Antibody

Binding Specificity: C-Term

Purpose: RAC2

Sequence: PQPTRQQKRA CSLL

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PQPTRQQKRACSLL, from the C Terminus of the protein sequence according to NP_002863.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Tonsil shows staining in mantle zone and some in the interfolicular area. Unserstained in germinal centre. Recommended concentration, 2-4 μg/mL.

Western Blot: Approx 22 kDa band observed in lysates of cell line Molt4 (calculated MW of 21.4 kDa according to NP_002863.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RAC2

Target: RAC2 (Ras-Related C3 Botulinum Toxin Substrate 2 (Rho Family, Small GTP Binding Protein Rac2) (RAC2))

Alternative Name: RAC2

Background: RAC2, ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2), EN-7, Ras-related C3 botulinum toxin substrate 2, rho family, small GTP binding protein Rac2, Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-

Gene ID: 5880

NCBI Accession: NP_002863

Pathways: RTK Signaling, VEGF Signaling