VAV2 antibody (Internal Region)

Catalog No. ABIN184702

This Goat Polyclonal antibody specifically detects VAV2 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for VAV2 antibody (Internal Region) (ABIN184702)

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VAV2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-VAV2 Antibody

Binding Specificity: Internal Region

Purpose: VAV2

Sequence: ETEAKYYRTL EDIEC

Specificity: This antobody is expected to recognise both reported isoforms (NP_001127870.1 and NP_003362.2).

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ETEAKYYRTLEDIEC, from the internal region of the protein sequence according to NP_001127870.1, NP_003362.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 100 kDa band observed in 293 lysate (predicted MW of 100 kDa according to NP_003362). Recommended for use at 1-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VAV2

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Alternative Name: VAV2

Background: VAV2, vav 2 oncogene, Oncogene VAV2, vav 2 guanine nucleotide exchange factor, Protein vav-2, oncogene VAV2

Gene ID: 7410, 22325

NCBI Accession: NP_001127870, NP_003362

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway