NQO1 antibody (C-Term)

Catalog No. ABIN184714

This Goat Polyclonal antibody specifically detects NQO1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for NQO1 antibody (C-Term) (ABIN184714)

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This NQO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-NQO1 Antibody

Binding Specificity: C-Term

Purpose: NQO1

Sequence: SIPTDNQIKA RK

Specificity: This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).

Cross-Reactivity: Dog, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SIPTDNQIKARK, from the C Terminus of the protein sequence according to NP_000894.1, NP_001020604.1, NP_001020605.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows specific staining of the glomeruli. Recommended concentration: 2-4 μg/mL.

Western Blot: Approx. 30 kDa band observed in lysates of cell lines U251 and HepG2 and in Human Kidney, Lung and Duodenum lysates, and approx. 30-32 kDa band in Rat Kidney, Lung and Duodenum lysates (calculated MW of 30.9 kDa according to Human NP_000894.1

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of HepG2 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NQO1

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Alternative Name: NQO1

Background: NQO1, NAD(P)H dehydrogenase, quinone 1, DTD, QR1, DHQU, DIA4, NMOR1, NMORI, diaphorase-4, diaphorase (NADH/NADPH), diaphorase (NADH/NADPH) (cytochrome b-5 reductase), NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1, DT-diaphorase, NAD(P)H menadione

Gene ID: 1728, 18104

NCBI Accession: NP_000894, NP_001020604, NP_001020605