BAG5 antibody (N-Term)

Catalog No. ABIN184771

This Goat Polyclonal antibody specifically detects BAG5 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for BAG5 antibody (N-Term) (ABIN184771)

Target: BAG5 (BCL2-Associated Athanogene 5 (BAG5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BAG5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-BAG5 Antibody

Binding Specificity: N-Term

Purpose: BAG5 (N Terminus)

Sequence: DMGNQHPSIS RLQ

Specificity: Variants (NP_004864.1, NP_001015048.1) encode the same isoform.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DMGNQHPSISRLQ-C, from the N Terminus of the protein sequence according to NP_001015049.1, NP_004864.1, NP_001015048.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 55+48 kDa bands observed in nuclear lysates of cell line HeLa (calculated MW of 56.0 kDa according to NP_001015049.1 and of 51.2 kDa according to NP_004864.1, NP_001015048.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BAG5

Target: BAG5 (BCL2-Associated Athanogene 5 (BAG5))

Alternative Name: BAG5

Background: BAG5, BCL2-associated athanogene 5, BAG-5, KIAA0873, BAG-family molecular chaperone regulator-5

Gene ID: 9529

NCBI Accession: NP_001015049, NP_004864, NP_001015048

Pathways: SARS-CoV-2 Protein Interactome