JUP antibody (C-Term)

Catalog No. ABIN184775

The Goat Polyclonal anti-JUP antibody has been validated for WB, IHC and ELISA. It is suitable to detect JUP in samples from Human, Mouse and Rat.

Quick Overview for JUP antibody (C-Term) (ABIN184775)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-JUP Antibody

Binding Specificity: C-Term

Purpose: Plakoglobin / Gamma-catenin

Sequence: GLRPPYPTAD HMLA

Specificity: This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GLRPPYPTADHMLA, from the C Terminus of the protein sequence according to NP_002221.1, NP_068831.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of cell lines A431 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7 kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: JUP

Background: JUP, GAMMA-CATENIN, gamma-catenin, junction plakoglobin, DP3, PDGB, PKGB, DPIII, ARVD12, CTNNG, OTTHUMP00000164735, catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80 kDa

Gene ID: 3728, 16480

NCBI Accession: NP_002221, NP_068831

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location