PRDM16 antibody (C-Term)

Catalog No. ABIN184809

Product Details anti-PRDM16 Antibody

Target: PRDM16 (PR Domain Containing 16 (PRDM16))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PRDM16 antibody is un-conjugated

Application: ELISA, Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS)

Purpose: PRDM16 / MEL1

Sequence: TSESGAFHPI NHL

Specificity: This antibody is expected to recognise both reported isoforms.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-TSESGAFHPINHL, from the C Terminus of the protein sequence according to NP_071397.2, NP_955533.1.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

ChIP: This product has been successfully used in ChIP on Mouse: Hondares E et al. (2011) Cell Transplant. 2011,20(8):1179-92

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PRDM16 antibody (ABIN184809)

Liu, Guan, Wen, Ma, Xu, Hu, Wei, Li, Wang, Xiang, Liao, Ji: "PRDM16 Inhibits Cell Proliferation and Migration via Epithelial-to-Mesenchymal Transition by Directly Targeting Pyruvate Carboxylase in Papillary Thyroid Cancer." in: Frontiers in cell and developmental biology, Vol. 9, pp. 723777, (2021) (PubMed).

Target Details for PRDM16

Target: PRDM16 (PR Domain Containing 16 (PRDM16))

Alternative Name: PRDM16 (PRDM16 Products)

Synonyms: PRDM16 antibody, wu:fc09g08 antibody, MEL1 antibody, PFM13 antibody, 5730557K01Rik antibody, C130091E20 antibody, csp1 antibody, mKIAA1675 antibody, mel1 antibody, PR/SET domain 16 antibody, PR domain containing 16 antibody, PRDM16 antibody, prdm16 antibody, Prdm16 antibody

Background: PRDM16, MEL1, KIAA1675, PR domain containing 16, transcription factor MEL1, MGC166915, PFM13, MDS1/EVI1-like, PR-domain zinc finger protein 16

Gene ID: 63976

NCBI Accession: NP_071397, NP_955533

Pathways: Stem Cell Maintenance, Brown Fat Cell Differentiation