CENPF antibody (C-Term)

Catalog No. ABIN184827

This anti-CENPF antibody is a Goat Polyclonal antibody detecting CENPF in ELISA and IHC. Suitable for Human.

Quick Overview for CENPF antibody (C-Term) (ABIN184827)

Target: CENPF (Centromere Protein F (CENPF))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CENPF antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CENPF Antibody

Binding Specificity: C-Term

Purpose: Centromere protein F / mitosin

Sequence: GLESKGSENC KVQ

Specificity: The immunizing peptide shows a K to N mismatch with the corresponding sequence of the NCBI-curated isoform NP_057427.3.

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: GLESKGSENCKVQ

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 3.75 μg/mL.

Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 358 kDa according to NP_057427. Therefore we cannot recommend an optimal concentration.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CENPF

Target: CENPF (Centromere Protein F (CENPF))

Alternative Name: CENPF

Background: CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K nuclear protein, centromere protein F, 350/400ka (mitosin), RP11-262H5.1, hcp-1, cent

Gene ID: 1063

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection