CENPF antibody (AA 2190-2270)

Catalog No. ABIN7227826

This Rabbit Polyclonal antibody specifically detects CENPF in IHC and IF. It exhibits reactivity toward Human.

Quick Overview for CENPF antibody (AA 2190-2270) (ABIN7227826)

Target: CENPF (Centromere Protein F (CENPF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CENPF Antibody

Binding Specificity: AA 2190-2270

Purpose: Rabbit Anti-CENPF Polyclonal Antibody

Specificity: The antibody detects endogenous levels of CENPF protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human CENPF protein at AA range: 2190-2270

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:50-300,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CENPF

Target: CENPF (Centromere Protein F (CENPF))

Alternative Name: CENPF

Background: Centromere protein F, CENP-F, AH antigen, Kinetochore protein CENPF, MitosinCENPF (Centromere Protein F) encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.

Molecular Weight: 353kD

Gene ID: 1063

UniProt: P49454

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection