PSMF1 antibody (C-Term)

Catalog No. ABIN184933

This Goat Polyclonal antibody specifically detects PSMF1 in WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PSMF1 antibody (C-Term) (ABIN184933)

Target: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PSMF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-PSMF1 Antibody

Binding Specificity: C-Term

Purpose: PI31 / PSMF1 (Isoform 1)

Sequence: DHLPPPGYDD MYL

Specificity: This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-DHLPPPGYDDMYL

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in lysates of cell line HEK293, approx 30-32 kDa in lysates of cell lines K562, NIH3T3, Neuro2a and KNRK, and approx. 35 kDa in lysates of cell line LNCaP (calculated MW of 29.8 kDa according to Human NP_006805.2 an

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry:A customer reported weak staining of A431 cells at

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PSMF1

Target: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Alternative Name: PSMF1

Background: PSMF1, PI31, proteasome (prosome, macropain) inhibitor subunit 1 (PI31), RP4-545L17.1, proteasome inhibitor hP131 subunit, proteasome inhibitor subunit 1

Gene ID: 9491, 228769

NCBI Accession: NP_006805

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA