TRIM54 antibody (C-Term)

Catalog No. ABIN184954

This Goat Polyclonal antibody specifically detects TRIM54 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for TRIM54 antibody (C-Term) (ABIN184954)

Target: TRIM54 (Tripartite Motif Containing 54 (TRIM54))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM54 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-TRIM54 Antibody

Binding Specificity: C-Term

Purpose: MURF3 / TRIM54

Sequence: GSAGPEEERP DGP

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GSAGPEEERPDGP, from the C Terminus of the protein sequence according to NP_115935.3, NP_912730.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75 μg/mL.

Western Blot: Experiments gave bands at approx 45 kDa and 38 kDa in Human Skeletal Muscle lysate after 1 μg/mL antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1 kDa according to NP_115935, and 38.6 kDa according to

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRIM54

Target: TRIM54 (Tripartite Motif Containing 54 (TRIM54))

Alternative Name: TRIM54

Background: TRIM54, MURF-3, tripartite motif-containing 54, RNF30, MURF, ring finger protein 30, muscle-specific RING-finger protein 3, muscle-specific RING-finger protein homolog, OTTHUMP00000122598

Gene ID: 57159

NCBI Accession: NP_115935, NP_912730