REV1 antibody (C-Term)

Catalog No. ABIN185012

The Goat Polyclonal anti-REV1 antibody has been validated for IHC and ELISA. It is suitable to detect REV1 in samples from Human.

Quick Overview for REV1 antibody (C-Term) (ABIN185012)

Target: REV1 (REV1, Polymerase (DNA Directed) (REV1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This REV1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-REV1 Antibody

Binding Specificity: C-Term

Purpose: REV1L

Sequence: VLQQTYGSTL KVT

Specificity: This antibody is expected to recognise both reported isoforms.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-VLQQTYGSTLKVT, from the C Terminus of the protein sequence according to NP_057400.1, NP_001032961.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. Recommended concentration, 4-6 μg/mL.

Western Blot: No signal obtained yet but low background observed in MOLT-4 lysates at up to 1.5 μg/mL. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for REV1

Target: REV1 (REV1, Polymerase (DNA Directed) (REV1))

Alternative Name: REV1

Background: REV1, REV1L, REV1-like (yeast), REV1, REV1 protein, REV1 (yeast homolog)- like, REV1 homolog (S. cerevisiae), FLJ21523, MGC163283, MGC26225, REV1- like

Gene ID: 51455, 56210

NCBI Accession: NP_057400, NP_001032961

Pathways: DNA Damage Repair, Regulation of Actin Filament Polymerization, Production of Molecular Mediator of Immune Response