REV1 antibody

Catalog No. ABIN7231002

This Rabbit Polyclonal antibody specifically detects REV1 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for REV1 antibody (ABIN7231002)

Target: REV1 (REV1, Polymerase (DNA Directed) (REV1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REV1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-REV1 Antibody

Purpose: Rabbit Anti-REV1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of REV1 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human REV1 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for REV1

Target: REV1 (REV1, Polymerase (DNA Directed) (REV1))

Alternative Name: REV1

Background: DNA repair protein REV1, Alpha integrin-binding protein 80, AIBP80, Rev1-like terminal deoxycytidyl transferaseREV1 encodes REV1, DNA Directed Polymerase with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA.

Molecular Weight: 137kD

Gene ID: 51455

UniProt: Q9UBZ9

Pathways: DNA Damage Repair, Regulation of Actin Filament Polymerization, Production of Molecular Mediator of Immune Response