FOXA1 antibody (C-Term)

Catalog No. ABIN185021

The Goat Polyclonal anti-FOXA1 antibody has been validated for WB, FACS, IF and ELISA. It is suitable to detect FOXA1 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for FOXA1 antibody (C-Term) (ABIN185021)

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXA1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Grade: Verified

Product Details anti-FOXA1 Antibody

Binding Specificity: C-Term

Purpose: FOXA1 / HNF3A

Sequence: GVYSRPVLNT S

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in nuclear lysates of cell line HepG2 and approx 50-55 kDa in Mouse Liver and in nuclear lysates of cell line MCF7 calculated MW of 49.1 kDa according to Human NP_004487.2 and 48.9 kDa according to Mouse NP_032285.2)

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-FOXA1 antibody (ABIN185021)

Bjerke, Pietrzak, Melhuish, Frierson, Paschal, Wotton: "Prostate cancer induced by loss of Apc is restrained by TGFβ signaling." in: PLoS ONE, Vol. 9, Issue 3, pp. e92800, (2014) (PubMed).

Target Details for FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Alternative Name: FOXA1

Background: FOXA1, HNF3A, forkhead box A1, TCF3A, MGC33105, hepatocyte nuclear factor 3, alpha

Gene ID: 3169, 15375, 25098

NCBI Accession: NP_004487

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis