Forkhead Box K2 antibody (C-Term)

Catalog No. ABIN185049

This anti-Forkhead Box K2 antibody is a Goat Polyclonal antibody detecting Forkhead Box K2 in WB, ELISA, IF and FACS. Suitable for Human.

Quick Overview for Forkhead Box K2 antibody (C-Term) (ABIN185049)

Target: Forkhead Box K2 (FOXK2)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Forkhead Box K2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Forkhead Box K2 Antibody

Binding Specificity: C-Term

Purpose: FOXK2 / ILF (isoform 1)

Sequence: TPPAAVREKG VQN

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TPPAAVREKGVQN, from the C Terminus of the protein sequence according to NP_004505.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in nuclear lysates of cell lines HEK293, HeLa and Jurkat (calculated MW of 69.1 kDa according to NP_004505.2.) This molecular weight is routinely observed by other sources and was successfully blocked by incubatio

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasmic/vesicles of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recom

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Forkhead Box K2

Target: Forkhead Box K2 (FOXK2)

Alternative Name: FOXK2

Background: ILF1, FOXK2, ILF, ILF-1, interleukin enhancer binding factor 1, cellular transcription factor ILF-1, forkhead box K2

Gene ID: 3607

NCBI Accession: NP_004505