MAML1 antibody (N-Term)

Catalog No. ABIN185074

This Goat Polyclonal antibody specifically detects MAML1 in ELISA, WB and IHC. It exhibits reactivity toward Human.

Quick Overview for MAML1 antibody (N-Term) (ABIN185074)

Target: MAML1 (Mastermind-Like 1 (MAML1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MAML1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-MAML1 Antibody

Binding Specificity: N-Term

Purpose: MAML1 / Mastermind

Sequence: VLPTCPMAEF ALPR

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence VLPTCPMAEFALPR, from the N Terminus of the protein sequence according to NP_055572.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration, 2.5-3.8 μg/mL.

Western Blot: Approx 110 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 108 kDa according to NP_055572.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MAML1

Target: MAML1 (Mastermind-Like 1 (MAML1))

Alternative Name: MAML1

Background: MAML1, mastermind-like 1 (Drosophila), Mam1, Mam-1, KIAA0200, mastermind homolog, mastermind-like 1

Gene ID: 9794

NCBI Accession: NP_055572

Pathways: Notch Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development