AKAP8 antibody (N-Term)

Catalog No. ABIN185207

The Goat Polyclonal anti-AKAP8 antibody has been validated for WB and ELISA. It is suitable to detect AKAP8 in samples from Human.

Quick Overview for AKAP8 antibody (N-Term) (ABIN185207)

Target: AKAP8 (A Kinase (PRKA) Anchor Protein 8 (AKAP8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AKAP8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-AKAP8 Antibody

Binding Specificity: N-Term

Purpose: AKAP8 / AKAP95

Sequence: DQGYGGYGAW SAG

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DQGYGGYGAWSAG-C, from the N Terminus of the protein sequence according to NP_005849.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Experiments gave a band at approx 100 kDa in Human Liver lysate after 1 μg/mL antibody staining. This band corresponds to the 95 kDa band reported in literature (PMID: 10601332). This protein has a calculated MW of 76.1 kDa according to NP_0058

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AKAP8

Target: AKAP8 (A Kinase (PRKA) Anchor Protein 8 (AKAP8))

Alternative Name: AKAP8

Background: AKAP8, AKAP95, DKFZp586B1222, A kinase (PRKA) anchor protein 8, A kinase anchor protein 95, A-kinase anchor protein 8, A-kinase anchor protein, 95 kDa

Gene ID: 10270, 56399, 116633

NCBI Accession: NP_005849

Pathways: SARS-CoV-2 Protein Interactome