BIM antibody (C-Term)

Catalog No. ABIN185253

This anti-BIM antibody is a Goat Polyclonal antibody detecting BIM in WB and ELISA. Suitable for Human.

Quick Overview for BIM antibody (C-Term) (ABIN185253)

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BIM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-BIM Antibody

Binding Specificity: C-Term

Purpose: BIM

Sequence: FNAYYARRLE K

Specificity: This antibody is expected to recognise at least reported isoforms Bim-alpha1 (BimABCD), Bim-alpha2 (BimACD) and Bim-alpha3 (BimAD).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-FNAYYARRLEK, from the C Terminus of the protein sequence according to NP_619528.1, NP_619529.1, NP_996886.1,.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 23 kDa band observed in lysates of cell line K562 (calculated MW of 18.5 kDa according to NP_619528.1). Recommended for use at 0.5-2 μg/mL. Primary incubated 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BIM

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Alternative Name: BCL2L11

Background: BCL2L11, BCL2-like 11 (apoptosis facilitator), BAM, BIM, BOD, bcl-2 interacting mediator of cell death, bcl-2 interacting protein Bim, bcl-2-like protein 11, bcl-2-related ovarian death agonist, bcl2-L-11, Bim-alpha1, Bim-alpha2, Bim-alpha3

Gene ID: 10018

NCBI Accession: NP_619528, NP_619529, NP_996886

Pathways: PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity