DLC1 antibody (N-Term)

Catalog No. ABIN185264

The Goat Polyclonal anti-DLC1 antibody has been validated for IHC, ELISA and IF. It is suitable to detect DLC1 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for DLC1 antibody (N-Term) (ABIN185264)

Target: DLC1 (Deleted in Liver Cancer 1 (DLC1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DLC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-DLC1 Antibody

Binding Specificity: N-Term

Purpose: DLC1 (Isoforms 1 and 3)

Sequence: SVAIRKRSWE EHC

Specificity: This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.2 and NP_079043.3 resp) but NOT isoform 2 (NP_006085.2).

Cross-Reactivity: Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SVAIRKRSWEEHC, from the N Terminus of the protein sequence according to NP_872584.2, NP_079043.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Mouse Adrenal Gland. Recommended concentration: 4 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U251 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-DLC1 antibody (ABIN185264)

Ko, Yeung, Wong, Chan, Poon, Ng, Yam: "Deleted in liver cancer 1 isoforms are distinctly expressed in human tissues, functionally different and under differential transcriptional regulation in hepatocellular carcinoma." in: Liver international : official journal of the International Association for the Study of the Liver, Vol. 30, Issue 1, pp. 139-48, (2009) (PubMed).

Target Details for DLC1

Target: DLC1 (Deleted in Liver Cancer 1 (DLC1))

Alternative Name: DLC1

Background: DLC1, HP, ARHGAP7, STARD12, FLJ21120, p122-RhoGAP, deleted in liver cancer 1, Rho-GTPase-activating protein 7, START domain containing protein 12, StAR-related lipid, transfer protein 12, StAR-related lipid transfer (START) domain containing 12, deleted

Gene ID: 10395, 50768

NCBI Accession: NP_872584, NP_079043

Pathways: Tube Formation, Positive Regulation of Endopeptidase Activity