PAD4 antibody (N-Term)

Catalog No. ABIN185308

This Goat Polyclonal antibody specifically detects PAD4 in IHC, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for PAD4 antibody (N-Term) (ABIN185308)

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PAD4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-PAD4 Antibody

Binding Specificity: N-Term

Purpose: PAD4 / PADI4

Sequence: AQGTLIRVTP EQPC

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence AQGTLIRVTPEQPC, from the N Terminus of the protein sequence according to NP_036519.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 4-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Expression of the protein seen in the nucleus and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PAD4

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Alternative Name: PADI4

Background: PADI4, PAD, PDI4, PDI5, PADI5, peptidyl arginine deiminase, type IV, protein-arginine deiminase, peptidyl arginine deiminase, type V, PAD4, HL-60 PAD, PADI-H protein

Gene ID: 23569

NCBI Accession: NP_036519