COMT antibody (N-Term)

Catalog No. ABIN185328

This anti-COMT antibody is a Goat Polyclonal antibody detecting COMT in WB, ELISA and FACS. Suitable for Human.

Quick Overview for COMT antibody (N-Term) (ABIN185328)

Target: COMT (Catechol-O-Methyltransferase (COMT))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This COMT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-COMT Antibody

Binding Specificity: N-Term

Purpose: COMT (N Terminus)

Sequence: GDTKEQRILN HVLQC

Specificity: This antibody is expected to recognise both reported isoforms S-COMT NP_009294.1 and MB-COMT NP_000745.1, NP_001128633.1, NP_001128634..

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence GDTKEQRILNHVLQC, from the N Terminus of the protein sequence according to NP_000745.1, NP_001128633.1, NP_001128634.1, NP_009294.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 25 kDa band observed in Human Liver and Ovary lysates and in MCF7 cell lysates, and approx. 25 kDa and 30 kDa in U251 cell lysate (calculated MW of 30.0 kDa according to NP_000745.1(MB COMT) and 24.5 kDa according to NP_009294.1 (S-COMT)

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for COMT

Target: COMT (Catechol-O-Methyltransferase (COMT))

Alternative Name: COMT

Background: COMT, catechol-O-methyltransferase, OTTHUMP00000197749, OTTHUMP00000197750

Gene ID: 1312

NCBI Accession: NP_000745, NP_001128633, NP_001128634, NP_009294

Pathways: Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome