FGFR1 antibody (C-Term)

Catalog No. ABIN185351

The Goat Polyclonal anti-FGFR1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect FGFR1 in samples from Human.

Quick Overview for FGFR1 antibody (C-Term) (ABIN185351)

Target: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FGFR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-FGFR1 Antibody

Binding Specificity: C-Term

Purpose: FGFR1

Sequence: CLPRHPAQLA NGGLKR

Specificity: This antibody is expected to recognise five isoforms (as represented by NP_075598.2, NP_056934.2, NP_075593.1, NP_075594.1, NP_075599.1).

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CLPRHPAQLANGGLKR, from the C Terminus of the protein sequence according to NP_075598.2, NP_056934.2, NP_075593.1, NP_075594.1, NP_075599.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of the enterocytic cytoplasm. Recommended concentration: 4-6 μg/mL. Paraffin embedded Human Kidney. Recommended concentration: 4 μg/mL.

Western Blot: Approx 75 kDa band observed in Human Breast lysates (calculated MW of 82.2 kDa according to NP_075593, 81.9 kDa according to NP_075594). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FGFR1

Target: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Alternative Name: FGFR1

Background: FGFR1, H2, H3, H4, H5, CEK, FLG, FLT2, KAL2, BFGFR, C-FGR, N-SAM, fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome), FLG protein, N-sam tyrosine kinase, tyrosylprotein kinase, protein-tyrosine kinase, FMS-like tyrosine

Gene ID: 2260

NCBI Accession: NP_075598, NP_056934, NP_075593, NP_075594, NP_075599

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, S100 Proteins