Acetylcholinesterase antibody (C-Term)

Catalog No. ABIN185357

The Goat Polyclonal anti-Acetylcholinesterase antibody has been validated for WB, ELISA, IF and FACS. It is suitable to detect Acetylcholinesterase in samples from Human.

Quick Overview for Acetylcholinesterase antibody (C-Term) (ABIN185357)

Target: Acetylcholinesterase (AChE)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Acetylcholinesterase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Acetylcholinesterase Antibody

Binding Specificity: C-Term

Purpose: ACHE

Sequence: QFDHYSKQDR CSDL

Specificity: This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in HepG2 and Jurkat cell lysates (calculated MW of 67.8 kDa according to NP_000656.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm, plasma membrane and nucleus of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomm

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Alternative Name: ACHE

Background: ACHE, YT, acetylcholinesterase (YT blood group), ARACHE, N-ACHE, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase, apoptosis-related acetylcholinesterase

Gene ID: 43

NCBI Accession: NP_000656, NP_001269378

Pathways: Skeletal Muscle Fiber Development