BID antibody (C-Term)
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- Target See all BID Antibodies
- BID (BH3 Interacting Domain Death Agonist (BID))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This BID antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- Purpose
- BID
- Sequence
- TYVRSLARNG MD
- Specificity
- This antibody is expected to recognise all three reported isoforms (as represented by NP_932070.1, NP_001187.1, NP_932071.1)
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-TYVRSLARNGMD, from the C Terminus of the protein sequence according to NP_932070.1, NP_001187.1, NP_932071.1.
- Isotype
- IgG
- Top Product
- Discover our top product BID Primary Antibody
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- Application Notes
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Western Blot: Approx 26+24 kDa bands observed in A431 cell lysates (calculated MW of 26.8 kDa according to NP_932070.1 and 22.0 kDa according to NP_001187.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:8000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- BID (BH3 Interacting Domain Death Agonist (BID))
- Alternative Name
- BID (BID Products)
- Synonyms
- FP497 antibody, 2700049M22Rik antibody, AI875481 antibody, AU022477 antibody, BID antibody, bid antibody, si:ch211-238n5.6 antibody, fp497 antibody, xbid antibody, DKFZp469E066 antibody, BH3 interacting domain death agonist antibody, BH3 interacting domain death agonist S homeolog antibody, BID antibody, Bid antibody, bida antibody, bid.S antibody, bid antibody
- Background
- BID, BH3 interacting domain death agonist , HGNC:1050, MGC15319, MGC42355 , BH3-interacting domain death agonist, Human BID coding sequence, apoptic death agonist, FP497, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, OTTHUMP00000196197, desmocoll
- Gene ID
- 637
- NCBI Accession
- NP_932070, NP_001187, NP_932071
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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