BID antibody (C-Term)

Catalog No. ABIN185380

This Goat Polyclonal antibody specifically detects BID in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for BID antibody (C-Term) (ABIN185380)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BID antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-BID Antibody

Binding Specificity: C-Term

Purpose: BID

Sequence: TYVRSLARNG MD

Specificity: This antibody is expected to recognise all three reported isoforms (as represented by NP_932070.1, NP_001187.1, NP_932071.1)

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TYVRSLARNGMD, from the C Terminus of the protein sequence according to NP_932070.1, NP_001187.1, NP_932071.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 26+24 kDa bands observed in A431 cell lysates (calculated MW of 26.8 kDa according to NP_932070.1 and 22.0 kDa according to NP_001187.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background: BID, BH3 interacting domain death agonist , HGNC:1050, MGC15319, MGC42355 , BH3-interacting domain death agonist, Human BID coding sequence, apoptic death agonist, FP497, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, OTTHUMP00000196197, desmocoll

Gene ID: 637

NCBI Accession: NP_932070, NP_001187, NP_932071

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity