PDHX antibody (C-Term)

Catalog No. ABIN185389

This Goat Polyclonal antibody specifically detects PDHX in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PDHX antibody (C-Term) (ABIN185389)

Target: PDHX (Pyruvate Dehydrogenase Complex, Component X (PDHX))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PDHX antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PDHX Antibody

Binding Specificity: C-Term

Purpose: Pyruvate dehydrogenase / PDHX

Sequence: KSFKANLENP IRLA

Specificity: This antibody is expected to recognise all reported isoforms.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KSFKANLENPIRLA, from the C Terminus of the protein sequence according to NP_003468.2, NP_001128496.1, NP_001159630.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in Human Pancreas lysate (calculated MW of 54.2 kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approx. 50 kDa is observed. This band is not observed in the non-transfecte

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PDHX

Target: PDHX (Pyruvate Dehydrogenase Complex, Component X (PDHX))

Alternative Name: PDHX

Background: PDHX, pyruvate dehydrogenase complex, component X, E3BP, OPDX, PDX1, proX, DLDBP, E3-binding protein, pyruvate dehydrogenase complex, lipoyl-containing component X, dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, pyruvate

Gene ID: 8050, 27402

NCBI Accession: NP_003468, NP_001128496, NP_001159630