RICTOR antibody (C-Term)

Catalog No. ABIN185390

The Goat Polyclonal anti-RICTOR antibody is suitable to detect RICTOR in samples from Human. It has been validated for IHC and ELISA.

Quick Overview for RICTOR antibody (C-Term) (ABIN185390)

Target: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RICTOR antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-RICTOR Antibody

Binding Specificity: C-Term

Purpose: RICTOR

Sequence: KQPIVDTSAE S

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-KQPIVDTSAES

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3 μg/mL.

Western Blot: Preliminary experiments gave an approx 38 kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in t

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RICTOR

Target: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Alternative Name: RICTOR

Background: RICTOR, Rictor, KIAA1999, AVO3, MGC39830, likely ortholog of mouse TORC2-specific protein AVO3 (S. cerevisiae), rapamycin-insensitive companion of mTOR, mAVO3, pianissimo, DKFZp686B11164, RPTOR independent companion of MTOR, complex 2, PIA, mAVO3, TORC2-s

Gene ID: 253260, 78757

NCBI Accession: NP_689969

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events