Glutathione Peroxidase 2 antibody (Internal Region)

Catalog No. ABIN185424

This anti-Glutathione Peroxidase 2 antibody is a Goat Polyclonal antibody detecting Glutathione Peroxidase 2 in ELISA and WB. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Glutathione Peroxidase 2 antibody (Internal Region) (ABIN185424)

Target: Glutathione Peroxidase 2 (GPX2)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Glutathione Peroxidase 2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Verified

Product Details anti-Glutathione Peroxidase 2 Antibody

Binding Specificity: Internal Region

Purpose: Glutathione peroxidase 2 (Int)

Sequence: SLDGEKVDFN

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SLDGEKVDFN, from the internal region of the protein sequence according to NP_002074.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 24 kDa band observed in Human Liver lysates (calculated MW of 21.9 kDa according to NP_002074.2). Recommended concentration: 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Glutathione Peroxidase 2 antibody (ABIN185424)

McGrath-Morrow, Lauer, Yee, Neptune, Podowski, Thimmulappa, OReilly, Biswal: "Nrf2 increases survival and attenuates alveolar growth inhibition in neonatal mice exposed to hyperoxia." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 296, Issue 4, pp. L565-73, (2009) (PubMed).

Naiki-Ito, Asamoto, Hokaiwado, Takahashi, Yamashita, Tsuda, Ogawa, Shirai: "Gpx2 is an overexpressed gene in rat breast cancers induced by three different chemical carcinogens." in: Cancer research, Vol. 67, Issue 23, pp. 11353-8, (2007) (PubMed).

Target Details for Glutathione Peroxidase 2

Target: Glutathione Peroxidase 2 (GPX2)

Alternative Name: GPX2

Background: GPX2, glutathione peroxidase 2 (gastrointestinal), HGNC:4554, GI-GPx, GSHPX-GI, gastrointestinal glutathione peroxidase 2, GPRP, GSHPx-2, glutathione peroxidase 2, glutathione peroxidase-related protein 2

Gene ID: 2877

NCBI Accession: NP_002074

Pathways: Thyroid Hormone Synthesis