Osteopontin antibody (C-Term)

Catalog No. ABIN185425

This Goat Polyclonal antibody specifically detects Osteopontin in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Osteopontin antibody (C-Term) (ABIN185425)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Osteopontin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Osteopontin Antibody

Binding Specificity: C-Term

Purpose: Osteopontin / SPP1

Sequence: DPKSKEEDKH LK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DPKSKEEDKHLK, from the C Terminus of the protein sequence according to NP_001035147.1, NP_000573.1, NP_001035149.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35 kDa band observed in lysates of cell line MOLT4. This band corresponds to the cleavage product from the 60 kDa glycoprotein (calculated MW of 33.8 kDa according to NP_000573.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: SPP1

Background: SPP1, HGNC:11255, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), BNSP, BSPI, ETA-1, MGC110940, OPN, Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), secreted phosphoprotein 1, SPP1/CALPHA1 fusion,

Gene ID: 6696, 20750

NCBI Accession: NP_001035147, NP_000573, NP_001035149

Pathways: Regulation of Cell Size