ERBB3 antibody (C-Term)

Catalog No. ABIN185461

This Goat Polyclonal antibody specifically detects ERBB3 in IHC, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for ERBB3 antibody (C-Term) (ABIN185461)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ERBB3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-ERBB3 Antibody

Binding Specificity: C-Term

Purpose: ERBB3 / HER3

Sequence: HSRLFPKANA QRT

Specificity: This antibody is expected to recognise isoform 1 (NP_001973.2) only.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HSRLFPKANAQRT, from the C Terminus of the protein sequence according to NP_001973.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Lung. Recommend concentration: 5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentratio

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: ERBB3

Background: ERBB3, HER3, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian), HGNC:3431, ErbB-3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3 , erbB-3, LCCS2, MGC88033, receptor tyrosine-protein kinase erbB-3, v-erb-b2 avian

Gene ID: 2065, 13867, 29496

NCBI Accession: NP_001973

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway