HRH2 antibody (C-Term)

Catalog No. ABIN185463

This anti-HRH2 antibody is a Goat Polyclonal antibody detecting HRH2 in WB, ELISA, IF and FACS. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for HRH2 antibody (C-Term) (ABIN185463)

Target: HRH2 (Histamine Receptor H2 (HRH2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HRH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-HRH2 Antibody

Binding Specificity: C-Term

Purpose: Histamine Receptor H2

Sequence: QEEKPLKLQV WSGTE

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QEEKPLKLQVWSGTE, from the C Terminus of the protein sequence according to NP_071640.1, NP_001124527.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in Human Tonsil lysates (calculated MW of 46.9 kDa according to Human NP_001354640.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-HRH2 antibody (ABIN185463)

Peng, Zhuang, He, Zhu, Wang: "Histamine excites neurons of the inferior vestibular nucleus in rats by activation of H1 and H2 receptors." in: Neuroscience letters, Vol. 541, pp. 87-92, (2013) (PubMed).

Target Details for HRH2

Target: HRH2 (Histamine Receptor H2 (HRH2))

Alternative Name: HRH2

Background: HRH2, histamine receptor H2, HGNC:5183, H2R, gastric receptor 1, OTTHUMP00000161242

Gene ID: 3274

NCBI Accession: NP_001124527, NP_001354640