HRH2 antibody (N-Term)

Catalog No. ABIN2856570

Product Details anti-HRH2 Antibody

Target: HRH2 (Histamine Receptor H2 (HRH2))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Cross-Reactivity: Human, Rat

Characteristics: Rabbit Polyclonal antibody to Histamine H2 Receptor (histamine receptor H2)
Histamine H2 Receptor antibody [N1], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histamine H2 Receptor. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NT2D1 , PC-3 , U87-MG , SK-N-SH

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.75 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-HRH2 antibody (ABIN2856570)

Adderley, Zhang, Breslin: "Involvement of the H1 Histamine Receptor, p38 MAP Kinase, Myosin Light Chains Kinase, and Rho/ROCK in Histamine-Induced Endothelial Barrier Dysfunction." in: Microcirculation (New York, N.Y. : 1994), Vol. 22, Issue 4, pp. 237-48, (2016) (PubMed).

Kurtz, Moor, Souza-Smith, Breslin: "Involvement of H1 and H2 receptors and soluble guanylate cyclase in histamine-induced relaxation of rat mesenteric collecting lymphatics." in: Microcirculation (New York, N.Y. : 1994), Vol. 21, Issue 7, pp. 593-605, (2015) (PubMed).

Target Details for HRH2

Target: HRH2 (Histamine Receptor H2 (HRH2))

Alternative Name: histamine receptor H2 (HRH2 Products)

Synonyms: H2R antibody, HH2R antibody, HRH2 antibody, histamine receptor H2 antibody, histamine receptor H2a antibody, histamine receptor H 2 antibody, HRH2 antibody, Hrh2 antibody, hrh2a antibody

Background: Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cell membrane, Multi-pass membrane protein

Molecular Weight: 40 kDa

Gene ID: 3274

UniProt: P25021