GDF15 antibody (C-Term)

Catalog No. ABIN185465

The Goat Polyclonal anti-GDF15 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect GDF15 in samples from Human.

Quick Overview for GDF15 antibody (C-Term) (ABIN185465)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GDF15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-GDF15 Antibody

Binding Specificity: C-Term

Purpose: GDF15

Sequence: QKTDTGVSLQ TYDD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QKTDTGVSLQTYDD, from the C Terminus of the protein sequence according to NP_004855.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 7 μg/mL.

Western Blot: Approx 35 kDa band observed in Human Prostate lysates and in lysates of cell lines A549, CaCo-2 and LNCaP (calculated MW of 34.1 kDa according to NP_004855.2). An additional band was seen at 48 kDa in some lysates and was successfully blocked

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15

Background: GDF15, PDF, MIC1, PLAB, MIC-1, NAG-1, PTGFB, GDF-15, growth differentiation factor 15, PTGF-beta, prostate differentiation factor, NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1

Gene ID: 9518

NCBI Accession: NP_004855

Pathways: SARS-CoV-2 Protein Interactome