Patched 1 antibody (C-Term)

Catalog No. ABIN185521

This Goat Polyclonal antibody specifically detects Patched 1 in ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse and has been mentioned in 2+ publications.

Quick Overview for Patched 1 antibody (C-Term) (ABIN185521)

Target: Patched 1 (PTCH1)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Patched 1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-Patched 1 Antibody

Binding Specificity: C-Term

Purpose: PTCH (C Terminus)

Sequence: ELQDVECEER PR

Specificity: This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1) encode the same isoform.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ELQDVECEERPR, from the C Terminus of the protein sequence according to NP_000255.1, NP_001077072.1, NP_001077071.1, NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Coretx). Recommended concentration: 8 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Golgi of HeLa and NIH3T3 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Patched 1 antibody (ABIN185521)

Abiria, Williams, Munden, Grover, Wallace, Lundberg, Valadez, Cooper: "Expression of Hedgehog ligand and signal transduction components in mutually distinct isocitrate dehydrogenase mutant glioma cells supports a role for paracrine signaling." in: Journal of neuro-oncology, Vol. 119, Issue 2, pp. 243-51, (2014) (PubMed).

Gerardo Valadez, Grover, Carter, Calcutt, Abiria, Lundberg, Williams, Cooper: "Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation." in: Cancer letters, Vol. 328, Issue 2, pp. 297-306, (2012) (PubMed).

Target Details for Patched 1

Target: Patched 1 (PTCH1)

Alternative Name: PTCH1

Background: PTCH1, PTCH, patched homolog (Drosophila), HGNC:9585, BCNS, HPE7, NBCCS, PTC, PTC1, patched, patched (Drosophila) homolog, patched homolog 1 (Drosophila), RP11-435O5.3, FLJ26746, FLJ42602, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b,

Gene ID: 5727

NCBI Accession: NP_000255, NP_001077072, NP_001077071, NP_001077073, NP_001077074, NP_001077075, NP_001077076

Pathways: Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation