Tryptophan Hydroxylase 2 antibody (Internal Region)

Catalog No. ABIN185533

This Goat Polyclonal antibody specifically detects Tryptophan Hydroxylase 2 in ELISA, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Tryptophan Hydroxylase 2 antibody (Internal Region) (ABIN185533)

Target: Tryptophan Hydroxylase 2 (TPH2)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Tryptophan Hydroxylase 2 Antibody

Binding Specificity: Internal Region

Purpose: Tryptophan hydroxylase 2 / TPH2

Sequence: NKPNSGKNDD KGNK

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NKPNSGKNDDKGNK, from the internal region of the protein sequence according to NP_775489.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Brain Stem shows staining of the cytoplasm of Purkinje cells and of neuron projections. Recommended concentration, 2-4 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Tryptophan Hydroxylase 2

Target: Tryptophan Hydroxylase 2 (TPH2)

Alternative Name: TPH2

Background: TPH2, tryptophan hydroxylase 2, HGNC:20692, FLJ37295, NTPH , neuronal tryptophan hydroxylase, ADHD7, MGC138871, MGC138872, tryptophan 5-monooxygenase 2

Gene ID: 121278

NCBI Accession: NP_775489