Tryptophan Hydroxylase 2 antibody (AA 16-29)

Catalog No. ABIN768607

Product Details anti-Tryptophan Hydroxylase 2 Antibody

Target: Tryptophan Hydroxylase 2 (TPH2)

Binding Specificity: AA 16-29

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: TPH2 (aa16-29)

Sequence: RGFSLDSAVP EEHQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence RGFSLDSAVPEEHQ-C, from the N Terminus of the protein sequence according to NP_775489.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Cryosections of Human Hypothalamus. Optimal dilution 1:5000.

Western Blot: Approx 60 kDa band observed in lysates of cell line HEK293 (calculated MW of 56.1 kDa according to NP_775489.2). Recommended concentration: 0.5-2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Tryptophan Hydroxylase 2

Target: Tryptophan Hydroxylase 2 (TPH2)

Alternative Name: TPH2 (TPH2 Products)

Synonyms: ADHD7 antibody, NTPH antibody, Ntph antibody, tphR antibody, wu:fq15a04 antibody, AU043594 antibody, tryptophan hydroxylase 2 antibody, tryptophan hydroxylase 2 (tryptophan 5-monooxygenase) antibody, TPH2 antibody, Tph2 antibody, tph2 antibody

Background: TPH2, tryptophan hydroxylase 2, ADHD7, FLJ37295, MGC138871, MGC138872, NTPH, neuronal tryptophan hydroxylase, tryptophan 5-hydroxylase 2, tryptophan 5-monooxygenase 2

Molecular Weight: 56.1kDa according to NP_775489.2

Gene ID: 121278, 216343, 317675

NCBI Accession: NP_775489