Tryptophan Hydroxylase 2 antibody (Internal Region)

Catalog No. ABIN768606

Product Details anti-Tryptophan Hydroxylase 2 Antibody

Target: Tryptophan Hydroxylase 2 (TPH2)

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Purpose: Tph2 (mouse)

Sequence: SLTQNKAIKS EDK

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SLTQNKAIKSEDK, from the internal region of the protein sequence according to NP_775567.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 85 kDa band in Mouse fetal Brain lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: In free-floating samples of Mouse Brain stem staining of Raphe nucleii serotonergic neurons can be seen, that is consistent with observation by different antibodies. Recommended concentration, 0.5-2μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Tryptophan Hydroxylase 2 antibody (ABIN768606)

Rahim, Meedeniya, Crane: "Central serotonergic neuron deficiency in a mouse model of Zellweger syndrome." in: Neuroscience, Vol. 274, pp. 229-41, (2014) (PubMed).

Target Details for Tryptophan Hydroxylase 2

Target: Tryptophan Hydroxylase 2 (TPH2)

Alternative Name: Tph2 (TPH2 Products)

Synonyms: ADHD7 antibody, NTPH antibody, Ntph antibody, tphR antibody, wu:fq15a04 antibody, AU043594 antibody, tryptophan hydroxylase 2 antibody, tryptophan hydroxylase 2 (tryptophan 5-monooxygenase) antibody, TPH2 antibody, Tph2 antibody, tph2 antibody

Background: Tph2, tryptophan hydroxylase 2, AU043594, MGC159133, Ntph, OTTMUSP00000022885, neuronal tryptophan hydroxylase, tryptophan 5-hydroxylase 2, tryptophan 5-monooxygenase 2

Molecular Weight: 85kDa

Gene ID: 216343

NCBI Accession: NP_775567