14-3-3 sigma/SFN antibody (Internal Region)

Catalog No. ABIN185539

This Goat Polyclonal antibody specifically detects 14-3-3 sigma/SFN in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for 14-3-3 sigma/SFN antibody (Internal Region) (ABIN185539)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-14-3-3 sigma/SFN Antibody

Binding Specificity: Internal Region

Purpose: 14-3-3 sigma / Stratifin

Sequence: DLHTLSEDSY KDST

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DLHTLSEDSYKDST, from the internal region of the protein sequence according to NP_006133.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4 μg/mL.

Western Blot: Approx 26-28 kDa band observed in Human, Mouse and Rat Skin lysates and in lysates of cell lines A431 and U2OS (calculated MW of 27.8 kDa according to Human NP_006133.1, Mouse NP_061224.2 and Rat XP_003750096.1). Recommended concentration: 0

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: SFN

Background: SFN, 14-3-3sigma, stratifin, HGNC:10773, YWHAS

Gene ID: 2810, 55948, 313017

NCBI Accession: NP_006133

Pathways: p53 Signaling, Myometrial Relaxation and Contraction