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CD14 antibody (Internal Region)

Name: CD14 antibody (Internal Region)
Brand: antibodies-online
SKU: ABIN185563
Price: 575.06 USD
Availability: InStock

This Goat Polyclonal antibody specifically detects CD14 in FACS, WB and ELISA. It exhibits reactivity toward Human.

(ABIN185563) Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0. (CD14 antibody (Internal Region))

FACS

(ABIN185563) (2 μg/mL) staining of Human Lymph node (A) + peptide (B) and Tonsil (C) + peptide (D) lysate (35 μg protein in RIPA buffer) Detected by chemiluminescence. (CD14 antibody (Internal Region))

ABIN185563 (1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). (CD14 antibody (Internal Region))

ABIN185563 (1µg/ml) staining of PBM lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. (CD14 antibody (Internal Region))

ABIN185563 (4µg/ml) staining of paraffin embedded Human Spleen. (CD14 antibody (Internal Region))

IHC

5 images

Catalog No. ABIN185563

Quick Overview for CD14 antibody (Internal Region) (ABIN185563)

Target

See all CD14 Antibodies

CD14

Reactivity

Human

Host

Goat

Clonality

Polyclonal

Conjugate

This CD14 antibody is un-conjugated

Application

Flow Cytometry (FACS), Western Blotting (WB), ELISA

Grade

Verified

Discover our top product CD14 Primary Antibody

Binding Specificity
59

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2

1

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Internal Region

Purpose

CD14

Sequence

KRVDADADPR QYAD

Cross-Reactivity

Human

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen

Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.

Isotype

IgG
anti-CD14 (CD14) (AA 54-83), (N-Term) antibody
CD14 Reactivity: Human FACS, WB, IF, IHC (p) Host: Rabbit Polyclonal RB14105 unconjugated

anti-CD14 (CD14) (AA 20-214) antibody
CD14 Reactivity: Human IHC, ELISA, ICC Host: Mouse Monoclonal 1H5D8 unconjugated

anti-CD14 (CD14) (AA 20-214) antibody
CD14 Reactivity: Human, Mouse FACS, WB, IHC, ELISA Host: Mouse Monoclonal 4B4F12 unconjugated

anti-CD14 (CD14) (AA 20-345) antibody
CD14 Reactivity: Human FACS, WB, IHC, ELISA, IF Host: Mouse Monoclonal 14G1F3 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human IHC, ELISA Host: Mouse Monoclonal 1A3B6 unconjugated

anti-CD14 (CD14) (AA 230-345) antibody
CD14 Reactivity: Human FACS, WB, IHC, ELISA Host: Mouse Monoclonal 1D1F8 unconjugated

anti-CD14 (CD14) (AA 25-148) antibody
CD14 Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal LPSR-2385 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP, Func Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) antibody
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) (AA 201-300) antibody
CD14 Reactivity: Human, Mouse, Rat, Sheep, Dog, Rabbit WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Application Notes

Western Blot: Approx 55 kDa band observed in Human Lymph node and Tonsil lysates (calculated MW of 40.1 kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form, and was successfully blocked by incubation with the im
Peptide ELISA: antibody detection limit dilution 1:128000.

Comment

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

CD14

Alternative Name

CD14

Background

CD14, CD14 antigen , HGNC:1628

Gene ID

929

NCBI Accession

NP_000582

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades