SCD antibody (C-Term)

Catalog No. ABIN185567

The Goat Polyclonal anti-SCD antibody has been validated for ELISA, IHC and FACS. It is suitable to detect SCD in samples from Human.

Quick Overview for SCD antibody (C-Term) (ABIN185567)

Target: SCD (Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SCD antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-SCD Antibody

Binding Specificity: C-Term

Purpose: Stearoyl-CoA desaturase

Sequence: RIKRTGDGNY KSG

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RIKRTGDGNYKSG, from the C Terminus of the protein sequence according to NP_005054.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver and Colon. Recommended concentration: 5 μg/mL.

Western Blot: Preliminary experiments gave an approx 30 kDa band in Human Adipose, Brain and Liver lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calc

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Flow Cytometry: Exclusively staining of HepG2 when mixed with Peripheral Blood Lymphocytes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SCD

Target: SCD (Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD))

Alternative Name: SCD

Background: SCD, SCD1, HGNC:10571, FADS5, PRO0998 , acyl-CoA desaturase, delta-9-desaturase, fatty acid desaturase, predicted protein of HQ0998, stearoyl-CoA desaturase

Gene ID: 6319

NCBI Accession: NP_005054

Pathways: Brown Fat Cell Differentiation