AGO1 antibody (Internal Region)

Catalog No. ABIN185581

This Goat Polyclonal antibody specifically detects AGO1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for AGO1 antibody (Internal Region) (ABIN185581)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AGO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-AGO1 Antibody

Binding Specificity: Internal Region

Purpose: EIF2C1 / AGO1

Sequence: KNASYNLDPY IQEF

Specificity: This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KNASYNLDPYIQEF, from the internal region of the protein sequence according to NP_036331.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 2.5 μg/mL.

Western Blot: In transfected HEK293 transiently expressing Human EIF2C1bands of approx. 110 kDa and 35 kDa are observed. The 110 kDa band is not observed in the non-transfected HEK293. The calculated molecular size is 97.2 kDa according to NP_036331.1. Recom

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Alternative Name: AGO1

Background: EIF2C1, eukaryotic translation initiation factor 2C, 1, AGO1, EIF2C, GERP95, Q99 , Golgi Endoplasmic Reticulum protein 95 kDa, argonaute 1, putative RNA-binding protein Q99

Gene ID: 26523

NCBI Accession: NP_036331

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization