HGS antibody (Internal Region)

Catalog No. ABIN185612

The Goat Polyclonal anti-HGS antibody is suitable to detect HGS in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for HGS antibody (Internal Region) (ABIN185612)

Target: HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HGS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-HGS Antibody

Binding Specificity: Internal Region

Purpose: HRS / HGS

Sequence: NRNYWEKKQE EARK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NRNYWEKKQEEARK, from the internal region of the protein sequence according to NP_004703.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows strong staining of Leydig cells. Recommended concentration: 1-2 μg/mL.

Western Blot: This antibody has been successfully used in Western Blot on Human: Clancy et al. (2020) https://doi.org/10.1101/2020.04.15.042291.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HGS

Target: HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))

Alternative Name: HGS

Background: HGS, hepatocyte growth factor-regulated tyrosine kinase substrate , HGNC:4897, HRS, ZFYVE8, human growth factor-regulated tyrosine kinase substrate

Gene ID: 9146, 15239, 56084

NCBI Accession: NP_004703

Pathways: EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Synaptic Vesicle Exocytosis, EGFR Downregulation