HSD3B1 antibody (C-Term)

Catalog No. ABIN185616

This anti-HSD3B1 antibody is a Goat Polyclonal antibody detecting HSD3B1 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for HSD3B1 antibody (C-Term) (ABIN185616)

Target: HSD3B1 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HSD3B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-HSD3B1 Antibody

Binding Specificity: C-Term

Purpose: HSD3B1

Sequence: DRHKETLKSK TQ

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DRHKETLKSKTQ, from the C Terminus of the protein sequence according to NP_000853.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa band observed in Human Placenta and Adrenal Gland lysates (calculated MW of 42.3 kDa according to NP_000853.1). Recommended concentration: 0.003-0.01 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HSD3B1

Target: HSD3B1 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1))

Alternative Name: HSD3B1

Background: HSD3B1, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, HGNC:5217, HSD3B, HSDB3, Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1

Gene ID: 3283

NCBI Accession: NP_000853

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis