HSD3B1 antibody (N-Term)

Catalog No. ABIN2777011

Cited in 1 publication. The Rabbit Polyclonal anti-HSD3B1 antibody (ABIN2777011) specifically detects HSD3B1 in WB. The antibody is reactive with Human, Goat and Monkey samples.

Quick Overview for HSD3B1 antibody (N-Term) (ABIN2777011)

Target: HSD3B1 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1))

Reactivity: Human, Goat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD3B1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HSD3B1 Antibody

Binding Specificity: N-Term

Purpose: HSD3B1 Antibody - N-terminal region

Sequence: TGWSCLVTGA GGFLGQRIIR LLVKEKELKE IRVLDKAFGP ELREEFSKLQ

Predicted Reactivity: Goat: 82%, Human: 100%, Monkey: 92%

Characteristics: This is a rabbit polyclonal antibody against HSD3B1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HSD3B1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP41821-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HSD3B1 antibody (ABIN2777011)

Mirkheshti, Park, Jiang, Cropper, Werner, Song, Chatterjee: "Dual targeting of androgen receptor and mTORC1 by salinomycin in prostate cancer." in: Oncotarget, Vol. 7, Issue 38, pp. 62240-62254, (2016) (PubMed).

Target Details for HSD3B1

Target: HSD3B1 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1))

Alternative Name: HSD3B1

Background:

Background Information: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Gene Name: Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1

Alternative Symbols: HSD3B, HSDB3, HSDB3A, SDR11E1, 3BETAHSD

Protein Name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Molecular Weight: 42kDa

Gene ID: 3283

NCBI Accession: NP_000853

UniProt: P14060

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis