NONO antibody (C-Term)

Catalog No. ABIN185629

This Goat Polyclonal antibody specifically detects NONO in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for NONO antibody (C-Term) (ABIN185629)

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This NONO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-NONO Antibody

Binding Specificity: C-Term

Purpose: NONO / p54NRB

Sequence: NRAAPGAEFA PNK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NRAAPGAEFAPNK, from the C Terminus of the protein sequence according to NP_031389.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen and Testis. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 60 kDa band observed in Mouse Brain lysates (calculated MW of 54.2 kDa according to Human NP_031389.3 and 54.5 kDa according to Mouse NP_075633.2). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NONO

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Alternative Name: NONO

Background: NONO, P54NRB , non-POU domain containing, octamer-binding , HGNC:7871, NMT55, NRB54, P54, Nuclear RNA-binding protein, 54-kD, non-POU-domain-containing, octamer-binding, non-Pou domain-containing octamer (ATGCAAAT) binding protein

Gene ID: 4841, 53610, 317259

NCBI Accession: NP_031389