Leptin Receptor antibody (Internal Region)

Catalog No. ABIN185639

The Goat Polyclonal anti-Leptin Receptor antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect Leptin Receptor in samples from Human, Mouse and Rat.

Quick Overview for Leptin Receptor antibody (Internal Region) (ABIN185639)

Target: Leptin Receptor (LEPR)

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Leptin Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Leptin Receptor Antibody

Binding Specificity: Internal Region

Purpose: Leptin Receptor

Sequence: TQDDIEKHQS DAG

Specificity: This antibody is expected to recognise all three reported isoforms (NP_002294.2, NP_001003679.1, NP_001003680.1).

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TQDDIEKHQSDAG, from the internal region of the protein sequence according to NP_002294.2, NP_001003679.1, NP_001003680.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 140-150 kDa band observed in Human Cerebellum, Mouse Foetal Brain and Rat Brain lysates (calculated MW of 132 kDa according to Human NP_002294.2 and 130.8 kDa according to Mouse NP_666258.2 and Rat NP_036728.1). This molecular weight is

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Leptin Receptor

Target: Leptin Receptor (LEPR)

Alternative Name: LEPR

Background: LEPR , leptin receptor , CD295, OBR , OB receptor, LeprB

Gene ID: 3953

NCBI Accession: NP_002294, NP_001003679, NP_001003680

Pathways: JAK-STAT Signaling, AMPK Signaling, Feeding Behaviour