Glutathione Peroxidase 1 antibody (Internal Region)

Catalog No. ABIN185652

This anti-Glutathione Peroxidase 1 antibody is a Goat Polyclonal antibody detecting Glutathione Peroxidase 1 in WB, IHC and ELISA. Suitable for Human and Pig.

Quick Overview for Glutathione Peroxidase 1 antibody (Internal Region) (ABIN185652)

Target: Glutathione Peroxidase 1 (GPX1)

Reactivity: Human, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Glutathione Peroxidase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Glutathione Peroxidase 1 Antibody

Binding Specificity: Internal Region

Purpose: Glutathione Peroxidase 1 (isoform1)

Sequence: REALPAPSDD ATA

Specificity: This antibody is expected to recognise isoform 1 ( NP_000572.2) only.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-REALPAPSDDATA, from the internal region of the protein sequence according to NP_000572.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows staining of microglia. Recommended concentration: 3-5 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 3.75 μg/mL. Frozen sections of Porcin Kidney shows staining of cyt

Western Blot: Approx 22 kDa band observed in Human Liver lysates (calculated MW of 22.1 kDa according to NP_000572.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Glutathione Peroxidase 1

Target: Glutathione Peroxidase 1 (GPX1)

Alternative Name: GPX1

Background: Glutathione peroxidase 1 , HGNC:4553, GPX1, GSHPX1, MGC14399, MGC88245

Gene ID: 2876

NCBI Accession: NP_000572

Pathways: Thyroid Hormone Synthesis, Sensory Perception of Sound, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome