COMT antibody (Internal Region)

Catalog No. ABIN185682

The Goat Polyclonal anti-COMT antibody (ABIN185682) specifically detects COMT in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for COMT antibody (Internal Region) (ABIN185682)

Target: COMT (Catechol-O-Methyltransferase (COMT))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This COMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-COMT Antibody

Binding Specificity: Internal Region

Purpose: COMT (internal)

Sequence: QDIIPQLKKK YDVD

Specificity: This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1)

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QDIIPQLKKKYDVD, from the internal region of the protein sequence according to NP_000745.1, NP_009294.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Brain (Cerebellum) shows staining of processes coming from the edge of the granular zone into the molecular layer. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Testis and Small Intestine. R

Western Blot: Approx 28 kDa band in Human Testis lysates (calculated MW of 30.0 kDa according to NP_000745.1 and 24.5 kDa according to NP_009294.1). Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for COMT

Target: COMT (Catechol-O-Methyltransferase (COMT))

Alternative Name: COMT

Background: COMT, catechol-O-methyltransferase

Gene ID: 1312

NCBI Accession: NP_000745, NP_009294

Pathways: Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome