DSCAM antibody (Internal Region)

Catalog No. ABIN185687

The Goat Polyclonal anti-DSCAM antibody has been validated for ELISA, WB, FACS and IF. It is suitable to detect DSCAM in samples from Human.

Quick Overview for DSCAM antibody (Internal Region) (ABIN185687)

Target: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DSCAM antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-DSCAM Antibody

Binding Specificity: Internal Region

Purpose: DSCAM

Sequence: DSWDSAQRTK DVSPQ

Specificity: This antibody is expected to recognise both reported isoforms (NP_001380.2 and NP_996770.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DSWDSAQRTKDVSPQ, from the internal region of the protein sequence according to NP_001380.2, NP_996770.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 200-220 kDa band observed in HepG2 cell lysates (calculated MW of 174 kDa according to NP_996770.1 ). This molecular weight is routinely observed by other sources. Recommended concentration: 2-3 μg/mL. Primary incubation 1 hour at room

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DSCAM

Target: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Alternative Name: DSCAM

Background: DSCAM, Down syndrome cell adhesion molecule , CHD2-42, CHD2-52 , human CHD2-52 down syndrome cell adhesion molecule

Gene ID: 1826, 13508, 171119

NCBI Accession: NP_001380, NP_001258463

Pathways: Regulation of Cell Size